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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene is observed in several tumors, which ends up in chromosome instability, and so might add to tumorigenesis. This protein was located to associate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a critical function in selling cell-cycle development during the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are described. Two supplemental splice variants ended up noted but detailed nucleotide sequence facts just isn't nonetheless offered.
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Blocking peptides are peptides that bind precisely on the concentrate on antibody and block antibody binding. These peptide typically has the epitope recognized through the antibody.
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This guidebook outlines additional possible optimization of mobile and immunoassay parameters to guarantee the absolute best outcomes are...
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This guidebook outlines more attainable optimization of mobile and immunoassay parameters to guarantee the very best final results are...
Antibodies certain to the blocking peptide not bind on the epitope over the focus on protein. By evaluating the staining through the blocked antibody compared to the antibody on your own, one can see which staining is specific.
Target Information and facts Cyclin E belongs into the remarkably conserved cyclin relatives, whose customers are characterized by a remarkable periodicity in protein abundance throughout the mobile cycle. Cyclins functionality as regulators of CDK kinases. Unique cyclins exhibit distinct expression and degradation styles which lead for the temporal coordination of each mitotic party. Cyclin E kinds a complex with and capabilities being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S transition. This protein accumulates for the G1-S stage boundary and is degraded as cells development by means of S section.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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